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deconvolution software nikon nis elements ar v5.02.0  (Nikon)

 
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    Nikon deconvolution software nikon nis elements ar v5.02.0
    Deconvolution Software Nikon Nis Elements Ar V5.02.0, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    deconvolution software nikon nis elements ar v5.02.0 - by Bioz Stars, 2026-03
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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D <t>widefield-deconvolution</t> microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).
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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D <t>widefield-deconvolution</t> microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).
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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy

    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy